Contact an Account ManagerImproved fluorometric method for quantitative HPLC analysis of methylated guanine derivatives in DNA
Summary
Type: HPLC
Application #: 107030
Catalog#: 720097.40 - MN HPLC Column, Nucleosil SA, 100A, 5um, 4x250mm
Detection: fluorescence, excitation 280nm, emission filter 350 - 470nm
Conditions: eluent: 0.4M ammonium phosphate, 5% methanol, pH 2.5
flow rate 0.7ml/min
temperature: ambient
Authors: Ratko TA
Source: J Chromatogr 324 (1985) 484 - 488
Details
Legend: Fig. 1. Elution profile of calf thymus DNA hydrolyzate alkylated in vitro with
NMU, Pyrim. Olig. = pyrimidine oligonucleotide.